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paper n a pt3 akt fluc ovap  (Addgene inc)


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    Addgene inc paper n a pt3 akt fluc ovap
    Paper N A Pt3 Akt Fluc Ovap, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plasmid+fluc-+n+-hibit/pUPD-pHXT7+(01-11)+(Plasmid+%23101686)/pmc11384939__mmc2-242-250-246
    Average 93 stars, based on 1 article reviews
    paper n a pt3 akt fluc ovap - by Bioz Stars, 2026-10
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    Article Title: m 6 A-driven SF3B1 translation control steers splicing to direct genome integrity and leukemogenesis.
    Article Snippet: Phoenix Ampho ATCC RRID: CVCL_H716 Experimental models: Organisms/strains Mouse: C57BL/6-Tg(Vav1-NUP98/HOXD13) G2Apla/J (NHD13) Jackson Laboratories CAT#010505 Mouse: NOD/SCID/g Jackson Laboratories CAT#005557 Mouse: C57/Bl6/SvJ Lund University N/A Oligonucleotides Oligonucleotides for qPCR analysis and genome editing in Table S9 This paper N/A Recombinant DNA pLCV.2 TRE_c-MYC EF1a_rtTA_P2A_PuroCrRED This paper N/A pLKO.1 TRC U6_shCTR hPGK_eGFP This paper N/A pLKO.1 TRC U6_shSF3B1 (hsa) hPGK_eGFP This paper N/A pLKO.1 TRC U6_shSF3B1 (mms) hPGK_eGFP This paper N/A Monocistronic SF3B1 5’UTR WT This paper N/A Monocistronic SF3B1 5’UTR WT ‘m6A-low’ This paper N/A Monocistronic SF3B1 5’UTR A88GMUT This paper N/A Monocistronic SF3B1 5’UTR A88GMUT ‘m6A-low’ This paper N/A Monocistronic SF3B1 5’UTR C89T MUT This paper N/A Monocistronic SF3B1 5’UTR uORF1MUT This paper N/A Monocistronic SF3B1 5’UTR uORF2MUT This paper N/A (Continued on next page) ll OPEN ACCESSArticle Molecular Cell 83, 1165–1179.e1–e11, April 6, 2023 e4 .. REAGENT or RESOURCE SOURCE IDENTIFIER pBABE HRasV12 Bellodi et al.74 N/A pBABE SF3B1 5’UTR WT-Fluc-3xMS2 Hygro This paper N/A pBABE SF3B1 5’UTR A88GMUT-Fluc-3xMS2 Hygro This paper N/A pcDNA3.1 (-) + FLAG-NLS-MS2-GFP Addgene #86827 psPAX2 Addgene #12260 pMD2.G Addgene #12259 ALKBH5-dCas9 Addgene #134783 TetO-FLAG-4EBP1MUT Hsieh et al.23 N/A Software and algorithms R R Core Team75 https://www.R-project.org/ SnapGene GSL Biotech LLC https://www.snapgene.com/ Prism 7 software Pad Software, Inc. N/A FACSDIVA BD Biosciences N/A Image J N/A https://imagej.nih.gov/ij/ STAR v2.5.2b Dobin et al.76 https://code.google.com/archive/p/rna-star/ DESeq2 Love et al.77 https://bioconductor.org/packages/release/ bioc/html/DESeq2.html rMAT v4.0.2 Shen et al.25 http://rnaseq-mats.sourceforge.net Samtools v0.9.1 Danacek et al.78 https://salmon.readthedocs.io/en/latest/ salmon.html#references Singscore Foroutan et al.79 https://www.bioconductor.org/packages/ release/bioc/html/singscore.html GSEA v4.0.1 Subramanian et al.80 https://www.gsea-msigdb.org/gsea/index.jsp bowtie2 Langmead et al.81 https://github.com/pzhaojohnson/RNA2Drawer/ blob/master/README.md RNA Framework suite Incarnato et al.82 http://www.rnaframework.com VastDB Center for Genomic Regulation, University of Toronto http://vastdb.crg.eu/wiki/Main_Page MATT Gohr and Irimia83 N/A ImageJ Schneider et al.84 https://imagej.nih.gov/ij/ Cutadapt v3.4 N/A https://journal.embnet.org/index.php/embnet journal/article/view/200 and https://github. com/marcelm/cutadapt Other BioComp gradient station BioComp N/A AriaIII cell sorter BD N/A BD LSR Fortessa BD N/A .. GloMax Explorer luminometer Promega N/A Nikon Eclipse 2000 light microscope Nikon N/A Zeiss 780 Confocal Laser Scanning Microscope Nikon N/A NextSeq 500 sequencer Illumina N/A UV Stratalinker 1800 Stratagene N/A ll OPEN ACCESS Article

    other:

    Article Title: Lysosomal cystine governs ferroptosis sensitivity in cancer via cysteine stress response.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Slc7a11 shRNA primer #1 (mouse): CGAACAAAAAAGCAGGCGGTACCGAATCAGCCT CGAGGCTGATTCGGTACCGCCTGCC IDT N/A Slc7a11 shRNA primer #2 (mouse): ACCGGGAGGGAAACTAAAAATGGAGCTCGAGCT CCATTTTTAGTTTCCCTCTTTTTTG IDT N/A Slc7a11 shRNA primer #2 (mouse): CGAACAAAAAAGAGGGAAACTAAAAATGGAGC TCGAGCTCCATTTTTAGTTTCCCTCC IDT N/A Slc7a11 shRNA primer #1 (human): ACCGGGGTGTTTCTGAGTAGTAATTACTCGAGTA ATTACTACTCAGAAACACCTTTTTTG IDT N/A Slc7a11 shRNA primer #1 (human): CGAACAAAAAAGGTGTTTCTGAGTAGTAATTA CTCGAGTAATTACTACTCAGAAACACCC IDT N/A AhR shRNA primer #1 (mouse): ACCGGTGAGGTGCCTGCTGGATAATTCTCGAGAA TTATCCAGCAGGCACCTCATTTTTTG IDT N/A AhR shRNA primer #1 (mouse): CGAACAAAAAATGAGGTGCCTGCTGGATAATTC TCGAGAATTATCCAGCAGGCACCTCAC IDT N/A AhR shRNA primer #2 (mouse): ACCGGCAGAGCTCTTTCCGGATAATACTCGA GTATTATCCGGAAAGAGCTCTGTTTTTTG IDT N/A AhR shRNA primer #2 (mouse): CGAACAAAAAACAGAGCTCTTTCCGGATAAT ACTCGAGTATTATCCGGAAAGAGCTCTGC IDT N/A pAtf4 (-1200bp) F: ATTGACTAGTACGCCTGGGCC AATCAGCTCGAC IDT N/A pAtf4 (-1000bp) F: ATTGACTAGTTCTCATGGGGCCTT TAGGACGAT IDT N/A pAtf4 (-800bp) F: ATTGACTAGTCATTTCTGCTTGCTG TCTGCCGG IDT N/A pAtf4 (-500bp) F: ATTGACTAGTGCGTTGCCTGCGACG CCGGCGCT IDT N/A pAtf4 (-400bp) F: ATTGACTAGTGCTCACCGGGGTCC CCGTGTCAT IDT N/A pAtf4 (-300bp) F: ATTGACTAGTCGTATTAGGACGCGA GGACAAGC IDT N/A pAtf4 (-200bp) F: ATTGACTAGTCACAATGGCCTTGGG CCCGCGTG IDT N/A pAtf4 (-100bp) F: ATTGACTAGTCCATCCAGGCTCTTC ACGAAATC IDT N/A pAtf4 (-400bp) R: ATGCGGATCCCCCGAGATGATTAA GCTAAGACA IDT N/A pAtf4 (-800bp) R: ATGCGGATCCGGCGGCGGCACGCCC TAAACCCG IDT N/A pAtf4 (-1000bp) R: ATGCGGATCCAGAAAAGTGCACTACT TTATAGG IDT N/A pAtf4 (0bp) R: ATGCGGATCCGTTGTGGGGCTTTGCTGG ATTCGAG IDT N/A Recombinant DNA pLJM1-Tmem192-mRFP-3xHA Addgene 134631 Firefly luciferase (pcDNA3.1) This study N/A (Continued on next page) Molecular Cell 83, 3347–3359.e1–e9, September 21, 2023 e4

    Article Title: MAPK14/p38α shapes the molecular landscape of endometrial cancer and promotes tumorigenic characteristics.
    Article Snippet: Pa02C ATCC N/A Pa03C ATCC N/A FaDU ATCC N/A KYSE-30 UNC Tissue Culture Facility N/A Oligonucleotides sgRNA sequences This paper Table S3 qRT-PCR primer This paper Table S3 Recombinant DNA FU-H2B-GFP-IRES-Puro Addgene Plasmid 69550 lentiCRISPRv2 DD-Cas9-BSD This paper N/A FLuc-mCh-puro Mann et al.113 N/A pLX313-TP53-WT Addgene Plasmid 118014 pLX313-TP53-P278A Addgene Plasmid 118015 Software and algorithms FlowJo FlowJo, LLC N/A ImageJ2 (Fiji) Rueden et al.114 N/A TRRUSTv2 Han et al.49 www.grnpedia.org/trrust Ingenuity pathway analysis Qiagen N/A Prism 9 GraphPad Software N/A BioRender https://www.biorender.com/ Living Image Software 4.7.4 Perkin Elmer N/A ZEN Zeiss N/A Gene Set Enrichment Analysis (GSEA) GSEA (Broad Institute) https://www.gsea-msigdb.org/ gsea/index.jsp 22 Cell Reports 44, 115104, January 28, 2025

    Article Title: Soluble Tim-3 serves as a tumor prognostic marker and therapeutic target for CD8 T cell exhaustion and anti-PD-1 resistance
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER human Tim3 primer 2 Reverse primer (50-30): CTGCTACTGCATTTGCCAATCCTG Beijing Tsingke Biotech Co., Ltd. N/A h-ADAM10 Forward primer (50-30): TGGTGGCACATTTTATGTTGAGC Beijing Tsingke Biotech Co., Ltd. N/A h-ADAM10 Reverse primer (50-30): AAGTTCTGGACCATTAGCAGC Beijing Tsingke Biotech Co., Ltd. N/A h-ADAM 17 Forward primer (50-30): GACTCTAGGGTTCTAGCCCAC Beijing Tsingke Biotech Co., Ltd. N/A h-ADAM 17 Reverse primer (50-30): GGAGACTGCAAACGTGAAACAT Beijing Tsingke Biotech Co., Ltd. N/A mIFN-g Forward primer (50-30): TGAGTATTGCCAAGTTTGAGGTCA Beijing Tsingke Biotech Co., Ltd. N/A mIFN-g Reverse primer (50-30): CGGCAACAGCTGGTGGAC Beijing Tsingke Biotech Co., Ltd. N/A mTNF-a Forward primer (50-30): AGTGACAAGCCTGTAGCCC Beijing Tsingke Biotech Co., Ltd. N/A mTNF-a Reverse primer (50-30): GAGGTTGACTTTCTCCTGGTAT Beijing Tsingke Biotech Co., Ltd. N/A mIL-2 Forward primer (50-30): GGAGCAGCTGTTGATGGACCTAC Beijing Tsingke Biotech Co., Ltd. N/A mIL-2 Reverse primer (50-30): AATCCAGAACATGCCGCAGAG Beijing Tsingke Biotech Co., Ltd. N/A m18S Forward primer (50-30): AGTTCCAGCACATTTTGCGAG Beijing Tsingke Biotech Co., Ltd. N/A m18S Reverse primer (50-30): TCATCCTCCGTGAGTTCTCCA Beijing Tsingke Biotech Co., Ltd. N/A h-bActin Forward primer (50-30): AGTTGCGTTACACCCTTTC Beijing Tsingke Biotech Co., Ltd. N/A h-bActin Reverse primer (50-30): CCTTCACCGTTCCAGTTT Beijing Tsingke Biotech Co., Ltd. N/A shCEACAM-1 sense: TGGGAGTCTACACACTAGATA Beijing Tsingke Biotech Co., Ltd. N/A shCEACAM-1 anti-sense: TATCTAGTGTGTAGACTCCCA Beijing Tsingke Biotech Co., Ltd. N/A sgCEACAM-1: TTCACGGGGCAAGCATACAG Beijing Tsingke Biotech Co., Ltd. N/A Recombinant DNA pT3/NICD1 Addgene (Gift from Xin Chen) #86500 pCMV(CAT)T7-SB100 Addgene (Gift from Zsuzsanna Izsvak) #34879 pT3-c-Myc Addgene (Gift from Xin Chen) #92046 pMXs-IRES-GFP Gift from Hongyan Wang N/A MSCV-pU6-(BbsI)-CcdB-(BbsI)Pgk-Puro-T2A-BFP Addgene cat#86457 psPAX2 Addgene cat#12260 pMD2G Addgene cat#12259 pHelper MiaoLingBio cat#P0243 pAAV-DJ MiaoLingBio cat#P2924 pCL-Eco Addgene cat#12371 pT3-AKT-Fluc This paper N/A pT3-AKT-Fluc-OVAp This paper N/A pT3-sTim-3 This paper N/A (Continued on next page) e3 Cell Reports Medicine 5, 101686, August 20, 2024 Article ll OPEN ACCESS

    Article Title: RNF10 and RIOK3 facilitate 40S ribosomal subunit degradation upon 60S biogenesis disruption or amino acid starvation.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays BCA Protein Assay Thermo Scientific (Pierce) Cat# 23225 CellTiter-Glo 2.0 Cell Viability Assay Promega Cat# G9243 Dual-Glo Luciferase Assay System Promega Cat# E2940 Deposited data 60S biogenesis knockdown proteomics (S3A-S3D, Figures 1F and 2A-C, 3A–3C, 6A; S1F; S2A) This paper PRIDE: PXD060355 Sucrose gradient siGTPBP4 proteomics (Figure 2F) This paper PRIDE: PXD060349 RNF10 KO with 60S biogenesis knockdown proteomics (Figures 3C and 5A, S3F-S3G, S6A) This paper PRIDE: PXD060309 eIF4A1 and GTPBP4 co-knockdown proteomics (Figures 4A and 4C; S4A) This paper PRIDE: PXD060375 RNF10 OE with eIF4A1 knockdown proteomics (Figures 4D, S4D) This paper PRIDE: PXD060334 GTPBP4 and NFX1/NFXL1 co-knockdown proteomics (Figures 5A, S5A-S5B) This paper PRIDE: PXD060331 eRQC factors and GTPBP4 co-knockdown proteomics (Figure 5B, S5C-S5E) This paper PRIDE: PXD060206 DBA-related proteins knockdown proteomics (Figures 5C, S5F) This paper PRIDE: PXD060207 40S biogenesis factor and GTPBP4 co-knockdown proteomics (Figures 5E-5F, S5G–S5H) This paper PRIDE: PXD060296 eS26 (RPS26) and GTPBP4 co-knockdown proteomics (Figures 5E-5F, S5G–S5H) This paper PRIDE: PXD060177 RIOK3 and GTPBP4 co-knockdown proteomics (Figures 6B–6D) This paper PRIDE: PXD060208 RNF10 OE proteomics (Figure S6B) This paper PRIDE: PXD060299 RIOK3 KO with GTPBP4 knockdown proteomics (Figures S6C–S6D) This paper PRIDE: PXD060294 RIOK3 variants rescue proteomics (Figures 7D, S7G) This paper PRIDE: PXD060301 Amino acid starvation in RNF10 KO and RIOK3 KO cell lines proteomics (Figure 7F, S7E–S7F, S7H–S7I) This paper PRIDE: PXD060308 Experimental models: Cell lines HEK293T ATCC Cat# CRL-3216 293Flp-In T-REx Thermo Fisher Scientific Cat# R78007 HAP1 Johnson et al.79 N/A OVTOKO JCRB Cell Bank Cat# JCRB1048 293FlpIn-RNF10KO Garshott et al.17 N/A 293FlpIn-FRT-Flag-HA-RNF10wt Garshott et al.17 N/A 293FlpIn-RIOK3KO_C4 This paper N/A 293FlpIn-Flag-HA-RIOK3-WT-siRes This paper N/A 293FlpIn-Flag-HA-RIOK3-DN-siRes This paper N/A 293FlpIn-Flag-HA-RIOK3-KM-siRes This paper N/A Oligonucleotides For oligonucleotides, see Table S1 N/A N/A Recombinant DNA pcDNA3-RLUC-IRES-FLUC Addgene Cat# 45642 pDONR-RIOK3-CDS hORFeome V8.1; Yang et al.77 N/A pSpCas9(BB)-2a-GFP plasmid (PX458) Addgene Cat# 48138 pcDNA3-RNF10-WT-UTR-RLUC-IRES-FLUC This paper N/A (Continued on next page) 18 Cell Reports 44, 115371, March 25, 2025

    Article Title: Soluble Tim-3 serves as a tumor prognostic marker and therapeutic target for CD8 + T cell exhaustion and anti-PD-1 resistance.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER human Tim3 primer 2 Reverse primer (50-30): CTGCTACTGCATTTGCCAATCCTG Beijing Tsingke Biotech Co., Ltd. N/A h-ADAM10 Forward primer (50-30): TGGTGGCACATTTTATGTTGAGC Beijing Tsingke Biotech Co., Ltd. N/A h-ADAM10 Reverse primer (50-30): AAGTTCTGGACCATTAGCAGC Beijing Tsingke Biotech Co., Ltd. N/A h-ADAM 17 Forward primer (50-30): GACTCTAGGGTTCTAGCCCAC Beijing Tsingke Biotech Co., Ltd. N/A h-ADAM 17 Reverse primer (50-30): GGAGACTGCAAACGTGAAACAT Beijing Tsingke Biotech Co., Ltd. N/A mIFN-g Forward primer (50-30): TGAGTATTGCCAAGTTTGAGGTCA Beijing Tsingke Biotech Co., Ltd. N/A mIFN-g Reverse primer (50-30): CGGCAACAGCTGGTGGAC Beijing Tsingke Biotech Co., Ltd. N/A mTNF-a Forward primer (50-30): AGTGACAAGCCTGTAGCCC Beijing Tsingke Biotech Co., Ltd. N/A mTNF-a Reverse primer (50-30): GAGGTTGACTTTCTCCTGGTAT Beijing Tsingke Biotech Co., Ltd. N/A mIL-2 Forward primer (50-30): GGAGCAGCTGTTGATGGACCTAC Beijing Tsingke Biotech Co., Ltd. N/A mIL-2 Reverse primer (50-30): AATCCAGAACATGCCGCAGAG Beijing Tsingke Biotech Co., Ltd. N/A m18S Forward primer (50-30): AGTTCCAGCACATTTTGCGAG Beijing Tsingke Biotech Co., Ltd. N/A m18S Reverse primer (50-30): TCATCCTCCGTGAGTTCTCCA Beijing Tsingke Biotech Co., Ltd. N/A h-bActin Forward primer (50-30): AGTTGCGTTACACCCTTTC Beijing Tsingke Biotech Co., Ltd. N/A h-bActin Reverse primer (50-30): CCTTCACCGTTCCAGTTT Beijing Tsingke Biotech Co., Ltd. N/A shCEACAM-1 sense: TGGGAGTCTACACACTAGATA Beijing Tsingke Biotech Co., Ltd. N/A shCEACAM-1 anti-sense: TATCTAGTGTGTAGACTCCCA Beijing Tsingke Biotech Co., Ltd. N/A sgCEACAM-1: TTCACGGGGCAAGCATACAG Beijing Tsingke Biotech Co., Ltd. N/A Recombinant DNA pT3/NICD1 Addgene (Gift from Xin Chen) #86500 pCMV(CAT)T7-SB100 Addgene (Gift from Zsuzsanna Izsvak) #34879 pT3-c-Myc Addgene (Gift from Xin Chen) #92046 pMXs-IRES-GFP Gift from Hongyan Wang N/A MSCV-pU6-(BbsI)-CcdB-(BbsI)Pgk-Puro-T2A-BFP Addgene cat#86457 psPAX2 Addgene cat#12260 pMD2G Addgene cat#12259 pHelper MiaoLingBio cat#P0243 pAAV-DJ MiaoLingBio cat#P2924 pCL-Eco Addgene cat#12371 pT3-AKT-Fluc This paper N/A pT3-AKT-Fluc-OVAp This paper N/A pT3-sTim-3 This paper N/A (Continued on next page) e3 Cell Reports Medicine 5, 101686, August 20, 2024

    Article Title: Taxanes trigger cancer cell killing in vivo by inducing non-canonical T cell cytotoxicity.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Organisms/strains Mus musculus FVB/NRj Janvier Labs N/A Mus musculus C57BL/6-Tg(TcraTcrb) 1100Mjb/J The Jackson laboratory 003831 Mus musculus NOD/SCID/IL2rg / The Netherlands Cancer Institute N/A Mus musculus FVB/NRj ROSA26mTmG The Netherlands Cancer Institute N/A Mus musculus K14-Cre; Brca1fl/fl; p53 fl/fl (KB1P) Described by Duarte et al., 2018.23 Obtained from the group of Jos Jonkers at the Netherlands Cancer Institute N/A Mus musculus MMTV-PyMT Described by Lin et al., 2003,24 obtained from The Jackson laboratory 022974 Biological samples K14-Cre; Brca1fl/fl; p53 fl/fl (KB1P) organoids Described by Duarte et al., 201823 N/A MMTV-PyMT organoids Described by Lin et al., 200324 N/A Organoids isolated from patients with breast cancer 13T; 27T; 38T; 62T; 100T; 169M; 36T Described by Sachs et al., 201833 and Dekkers et al., 202134 N/A Healthy human breast organoids m12 Described by Sachs et al., 201833 and Dekkers et al., 202134 N/A Organoids isolated from patients with NSCLC or CRC Described by Dijkstra et al., 201840 and Cattaneo et al., 202039 N/A T cells isolated from patients with NSCLC or CRC Described by Dijkstra et al., 201840 and Cattaneo et al., 202039 N/A Recombinant DNA ABCB1 ORF The Netherlands Cancer Institute See Annex 2 for map plv-OVA-mPlum Gift from the Peeper lab, the Netherlands Cancer Institute N/A pLenti CMV Puro LUC Addgene w168–1; Plasmid #17477 psPAX2 Addgene 12260 PMD2.G Addgene 12259 Software and algorithms FlowJo software 10.6.1 BD Biosciences https://www.flowjo.com/ Fiji/ImageJ https://imagej.net 2.1.0/1.53h MaxQuant software 1.6.17.0 https://www.maxquant.org/ N/A MetaMorph 7.10 software Molecular Devices N/A Prism GraphPad 9.0.0 (86) Other Oasis HLB plate Waters WA31764.66 Ultimate 3000 Thermo Fisher Scientific N/A 70 mm cell strainer Corning 352350 0.22 mm cell strainer Merck Millipore SLGS033SS Amilcon Ultra-15 10k column Merck Millipore UFC905024 Amilcon Ultra 2mL 10k column Merck Millipore UFC201024 Glass-bottom 96-well plate Greiner bio-one 655076 24-well ultra-low adherence cluster polystyrene culture dish Corning 4527 m-Precolumn, 300mm i.d. x 5mmC18 PepMap100, 5 mm, 100 Å Thermo Fisher Scientific 160454 Zeiss Axioskop2 Plus microscope Carl Zeiss Microscopy, Jena, Germany N/A inverted research microscope Nikon Eclipse Ti-E Nikon N/A (Continued on next page) e3 Cancer Cell 41, 1170–1185.e1–e12, June 12, 2023



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    Effect of Honokiol on NF-κB activation in N. gonorrhoeae -infected macrophages. ( A and B ) Macrophages were initially incubated with either honokiol or a control vehicle for 0.5 hours. Subsequently, they were infected with N. gonorrhoeae for an additional 4 hours. Western blotting was employed to analyze the phosphorylation levels of two proteins, namely IKK-α/β ( A ) and IκB-α ( B ), in the cell lysates. ( C ) J-Blue cells were incubated with honokiol or the control vehicle for 0.5 hours and then infected with N. gonorrhoeae for an additional 24 hours. The transcriptional activity of NF-κB was assessed using an NF-κB reporter assay. The NF-κB reporter assay data is presented as fold change compared to the control group, represented as mean ± SD of results from three separate experiments. The Western blotting images presented here depict individual experiments, while the histogram provides quantification expressed as fold change compared to the control group, represented as mean ± SD across these three experiments.

    Journal: Journal of Inflammation Research

    Article Title: Honokiol Exhibits Anti-NLRP3 Inflammasome and Antimicrobial Properties in Neisseria gonorrhoeae -Infected Macrophages

    doi: 10.2147/JIR.S454221

    Figure Lengend Snippet: Effect of Honokiol on NF-κB activation in N. gonorrhoeae -infected macrophages. ( A and B ) Macrophages were initially incubated with either honokiol or a control vehicle for 0.5 hours. Subsequently, they were infected with N. gonorrhoeae for an additional 4 hours. Western blotting was employed to analyze the phosphorylation levels of two proteins, namely IKK-α/β ( A ) and IκB-α ( B ), in the cell lysates. ( C ) J-Blue cells were incubated with honokiol or the control vehicle for 0.5 hours and then infected with N. gonorrhoeae for an additional 24 hours. The transcriptional activity of NF-κB was assessed using an NF-κB reporter assay. The NF-κB reporter assay data is presented as fold change compared to the control group, represented as mean ± SD of results from three separate experiments. The Western blotting images presented here depict individual experiments, while the histogram provides quantification expressed as fold change compared to the control group, represented as mean ± SD across these three experiments.

    Article Snippet: The NF-κB reporter cell line J-Blue was established from J774A.1 macrophage through stable transfection using an NF-κB-inducible reporter plasmid (pnifty2-seap), which was purchased from InvivoGen (Carlsbad, CA).

    Techniques: Activation Assay, Infection, Incubation, Control, Western Blot, Activity Assay, Reporter Assay